Comparison of T7- and Lac-Based Systems for the Periplasmic Expression of Human Granulocyte Macrophage Colony Stimulating Factor in Escherichia coli
نویسندگان: ثبت نشده
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The Expression of Human Granulocyte Macrophage Colony Stimulating Factor by Heat-Induction in Escherichia coli
A self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pL promoter, was constructed. The designed 5024 base pair expression plasmid contained a heat sensitive repressor cI857 coding gene to regulate the function of ?pL promoter under heat shock induction. Using the constructed vector,...
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a self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pl promoter, was constructed. the designed 5024 base pair expression plasmid contained a heat sensitive repressor ci857 coding gene to regulate the function of ?pl promoter under heat shock induction. using the constructed vector,...
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عنوان ژورنال
دوره 1 شماره 2
صفحات 101- 108
تاریخ انتشار 2003-04-01
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